Media Farmasi Jurnal Ilmu Farmasi Vol 20 No 2 2023

Title	Media Farmasi Jurnal Ilmu Farmasi Vol 20 No 2 2023
Edition	Vol 20 No 2 2023
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ISBN/ISSN
Author(s)
Subject(s)
Classification
Series Title
GMD	Karya Tulis Ilmiah
Language	Indonesia
Publisher	Universitas Ahmad Dahlan
Publishing Year	2023
Publishing Place	Jogjakarta
Collation
Abstract/Notes	Honey is a natural ingredient that Indonesian people widely use to maintain a healthy body. One of the benefits of honey is as an antioxidant. This antioxidant activity is influenced by the producing bee, the source plant, and the producing area. Antioxidants are chemicals that stop free radicals from oxidizing and causing harm or destruction. This research proposes to ascertain the antioxidant activity of Sumbawa honey from West Nusa Tenggara's ethanol extract, fractions of n-hexane, ethyl acetate, and water. Honey samples were collected and dried using freeze- drying, then macerated with 70% ethanol. The viscous extract obtained was then fractionated using a liquid-liquid extraction method using n- hexane, ethyl acetate, and ethanol-water as solvents so that the n-hexane (NF), ethyl acetate (EF), and water (WF) fractions were obtained. The ABTS technique was applied to assess the antioxidant activity of each sample, and Trolox was used as a reference. Antioxidant activity test using a UV-Vis spectrophotometer at a wavelength of 734 nm and operating time of 25th minutes. The results obtained were ethanol extract, NF, EF, and AF had IC50 values respectively 96.054; 106.953; 70.206; and 101.649 g/mL. That indicates that the ethyl acetate fraction has the strongest antioxidant activity. Comparatively, the antioxidant activity of Trolox has an IC50 value of 18.463 mg/ml. Trolox, a substance in the extremely strong category, has been able to outperform the antioxidant activity of the ethyl acetate fraction.
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