Biodiversitas Jurnal Of Biological Diversity Sebelas Maret University Volume 22, Number 4, April 2021

Title	Biodiversitas Jurnal Of Biological Diversity Sebelas Maret University Volume 22, Number 4, April 2021
Edition	Volume 22, Number 4, April 2021
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Subject(s)	Biological
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Series Title
GMD	Karya Tulis Ilmiah
Language	Indonesia
Publisher	The Society For Indonesia Biodiversity
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Publishing Place	Surakarta
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Abstract/Notes	DNA barcoding of the tidal swamp rice (Oryza sativa) landraces from South Kalimantan, Indonesia DINDIN HIDAYATUL MURSYIDIN1,♥ , YUDHI AHMAD NAZARI2 , BADRUZSAUFARI1 , MUHAMMAD RIDHO DINTA MASMITRA1 1Laboratory of Genetics and Molecular Biology, Faculty of Mathematics and Natural Sciences, Universitas Lambung Mangkurat. Jl. A. Yani Km. 36, Banjarbaru 70714, South Kalimantan, Indonesia. Tel.: +62-511-4773112, email: dindinhm@gmail.com. 2Department of Agroecotechnology, Faculty of Agriculture, Universitas Lambung Mangkurat. Jl. A. Yani Km. 36, Banjarbaru 70714, South Kalimantan, Indonesia Manuscript received: 16 January 2021. Revision accepted: 3 March 2021. Abstract. Mursyidin DH, Nazari YA, Badruzsaufari, Masmitra MRD. 2021. DNA barcoding of the tidal swamp rice (Oryza sativa) landraces from South Kalimantan, Indonesia. Biodiversitas 22: 1593-1599. The tidal swamp rice (Oryza sativa L.) landraces of the South Kalimantan, Indonesia, has been known for hundred years ago with a better adaptation to the local conditions, such as acidity, salinity, and metals contamination. However, the genetic insight of these landraces has not fully understood. Here, the rbcL region of tidal swamp rice from this region was successfully sequenced, aligned, analyzed, and deposited into the GenBank with accession numbers of MT818188–MT818201. The multiple alignments of these sequences showing a barcoding motif with eight mutation or polymorphic sites with both substitutions (transition-transversion) and indels (insertion-deletion). Based on this marker, the tidal swamp rice has a low genetic diversity, only 0.086. However, the UPGMA and maximum likelihood (ML) analyses revealed that this germplasm was grouped into five and twoclusters or clades, respectively. In this case, Bayar Putih is closely related to Siam Panangah and farthest from Lemo. This information might help to develop conservation and breeding programs of the tidal swamp rice landraces in the future. Keywords: Breeding program, DNA barcoding, genetic diversity, rice, tidal swamp area
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