Biodiversitas Jurnal Of Biological Diversity Sebelas Maret University Volume 21, Number 9, November 2020

Title	Biodiversitas Jurnal Of Biological Diversity Sebelas Maret University Volume 21, Number 9, November 2020
Edition	Volume 21, Number 9, November 2020
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ISBN/ISSN
Author(s)
Subject(s)	Biological
Classification
Series Title
GMD	Karya Tulis Ilmiah
Language	Indonesia
Publisher	The Society For Indonesia Biodiversity
Publishing Year
Publishing Place	Surakarta
Collation
Abstract/Notes	Inter-primer binding site (iPBS) markers reveal the population genetic diversity and structure of tropical climbing Cissampelopsis (Asteraceae) in Thailand ONGKARN VANIJAJIVA1 , PIMWADEE PORNPONGRUNGRUENG2,♥ 1Faculty of Science and Technology, Phranakhon Rajabhat University. 9 Chaeng Watthana Rd, Anusawari, Bang Khen, Bangkok 10220, Thailand 2Applied Taxonomic Research Center, Department of Biology, Faculty of Science, Khon Kaen University. Tambon Sila, Khon Kaen 40002, Thailand. Tel.: +66-43-009700 ext. 42177, Fax.: +61643-364169, email: ppimwa@kku.ac.th Manuscript received: 23 May 2020. Revision accepted: 3 August 2020. Abstract. Vanijajiva O, Pornpongrungrueng P. 2020. Inter-primer binding site (iPBS) markers reveal the population genetic diversity and structure of tropical climbing Cissampelopsis (Asteraceae) in Thailand. Biodiversitas 21: 3919-3928. Cissampelopsis is a small climbing tropical Asian genus of Asteraceae-Senecioneae. In Thailand, the genus is represented by two species, C. corifolia and C. Volubilis, distributed through the mountain evergreen forest. Study on the genetic diversity and structure of populations of both Cissampelopsis species provide better understanding of the biology and pattern of species diversification in the genus. To identify the genetic diversity, we used the inter-primer binding site (iPBS) retrotransposon system, in 96 accessions of Cissampelopsis species collected from different regions in Thailand. A total of 120 iPBS bands were scored as presence⁄ absence characters. Results from UPGMA and PCoA analyses indicated that C. corifolia and C. volubilis are different species. Genetic diversity and genetic differentiation among and within populations of C. volubilis is higher than C. corifolia. Molecular Variance (AMOVA) analysis of both species indicated that the genetic variance value within populations is higher than among populations of each species. Bayesian model- based STRUCTURE analysis detected two gene pools for both Cissampelopsis and showed admixture within individuals. Differences among the two Cissampelopsis species, in total diversities and levels of population differentiation, indicated that the genetic structure of Cissampelopsis populations are congruent with long-lived perennial habit with regional distribution, even for congeneric species, may vary considerably. This study suggests the effectiveness of the iPBS marker system to estimate the population genetic diversity and structure of Cissampelopsis genotypes. Keywords: Cissampelopsis, iPBS, genetic diversity, population structure, Thailand
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