Fakultas Farmasi Unmul Primary Author mixed material bibliography Samarinda Universitas Mulawarman 2021 monographic Vol. 5 No. 3 Januari-Juni (2021) id Indonesia

Karya Tulis Ilmiah

Abstract The genus Eucalyptus has been used in African traditional medicine for the treatment of cardiovascular diseases and diabetes. In this work, chloroform and methanol extracts from the leaves of Eucalyptus citriodora was investigated for their phytochemicals, cytotoxicity and antioxidant potentials. Phytochemical screening of the extracts showed the presence of alkaloids, flavonoids, phenols, reducing sugar and steroids in the methanol extract, while the chloroform extract presence of these phytochemicals was observed with exception of reducing sugar. IR absorptions of the extract supported the presence of these phytochemicals by revealing the bands 3335 cm-1 (O-H), 1613, 1480 cm-1 (C=C) and 1721 cm-1 (C=O). Evaluation of the cytotoxicity of the methanol extract using brine shrimp assay, suggested that, the extract was not toxic with LC50 value of 1.64 mg/mL. Antioxidant potentials of both chloroform and methanol extracts were determined using phenolic content quantification and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging. Methanol extract (37.32 μg TAE/mg) had higher phenolic content than the chloroform extract (12.09 μg TAE/mg). The radical scavenging potentials of the extracts recorded percent inhibitions (1000 μg/mL) of 51.33%, 93.05%, 94.43% and 97.80% for chloroform extract, methanol extract, butylated hydroxytoluene and ascorbic acid, respectively. These results showed that E. citriodora might contain promising antioxidant agents. Keywords: Eucalyptus, phytochemicals, brine shrimp, antioxidant farmasi PERPUSTAKAAN SEKOLAH TINGGI ILMU KESEHATAN SAMARINDA REPOSITORY JN0263 Perpus.Akfarsam 051 FAK j Journal Of Tropical Pharmacy and Cemistry Vol. 5 No. 3 Januari-Juni (2021) 1543 2022-03-18 09:37:48 2022-03-18 09:41:07 machine generated