Indonesia J.Pharm Primary Author mixed material bibliography Jogjakarta Universitas Gadjah Mada 2017 monographic Vol 28 no 4 October-Desember 2017 id Indonesia

Karya Tulis Ilmiah

ABSTRACT The reference method for simultaneous analysis of drugs is chromatography, however, this technique is expensive, complex, and needs excessive sample preparation; therefore, some simple methods like UV spectroscopy is proposed. Assisted with multivariate calibration, it is possible to analyze drugs using UV spectroscopy without prior separation. This study is intended to use UV spectroscopy coupled with multivariate calibration of partial least square (PLS) for simultaneous analysis of paracetamol (PCT), propyphenazone (PROPI), and caffeine (CAFF) in tablet dosage form. The calibration model was prepared by developing a series 20 mixture of PCT, PROPI and CAFF with certain composition randomly and its absorbance was measured at wavelength of 220-313nm with an interval of 3nm. The performance of calibration model was assessed by coefficient of determination (R2 ), root mean square error of calibration (RMSEC) and root mean square error of cross validation (RMSECV). The R2 values for the correlation between actual values of PCT, PROPI and CAFF and predicted values using UV-spectroscopy combined with PLS were 0.9994; 0.9878; and 0.9919, respectively. The calibration errors expressed with RMSEC were 0.027%, 0.082% and 0.043% for PCT, PROPI and CAFF, respectively. While, during cross validation using “leave one out” technique, RMSECV values obtained were 0.062%, 0.095% and 0.982%, respectively for PCT, PROPI and CAFF. The level of drugs obtained were 226.76±14.49 mg/tablet (equivalent to 90.70% from labeled claim) for PCT, 135.74±11.23 mg/tablet (equivalent to 90.49% from labeled claim) for PROPI and 51.69±2.35 mg/tablet (equivalent to 103.38% from labeled claim) for CAFF. Keywords: UV-spectrophotometry, multivariate calibration, partial least square, paracetamol, caffeine, and Propyphenazone. farmasi PERPUSTAKAAN SEKOLAH TINGGI ILMU KESEHATAN SAMARINDA REPOSITORY JN0120 Perpus.Akfarsam (Jurnal Nasional) 051 MOR j Indonesia Journal of Pharmacy Vol 28 no 4 October-Desember 2017 1491 2022-03-17 08:57:35 2022-03-25 10:07:27 machine generated