Indonesia Journal of Pharmacy Vol 27 no 3 Juli-September 2016

Title	Indonesia Journal of Pharmacy Vol 27 no 3 Juli-September 2016
Edition	Vol 27 no 3 Juli-September 2016
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ISBN/ISSN
Author(s)	Faculty of Farmacy UGM - Personal Name
Subject(s)	farmasi
Classification
Series Title
GMD	Karya Tulis Ilmiah
Language	Indonesia
Publisher	Universitas Gadjah Mada
Publishing Year	2016
Publishing Place	Jogjakarta
Collation
Abstract/Notes	ABSTRACT Self-Nanoemulsifying Drug Delivery System (SNEDDS) has potential to be developed for oral protein delivery because it is free from water, hence preserving the stability of protein, protecting protein from enzymatic degradation, and enhancing the protein permeability in the gastrointestinal tract (GIT). However, protein-based SNEDDS formulation is challenging due to low solubility property of protein in oil, which is towards zero. This present study aimed to obtain the most compatible SNEDDS for protein using HLB approach. Bovine serum albumin (BSA) was used as a protein model. A number of 78 formulas with HLB ranging between 11 and 15 were screened to acquire stable SNEDDS composition without the presence of phase separation. Of 13 stable formulas, two were selected (F30 and F45) with HLB 15, and then loaded with BSA. Physical characteristics of both formulas were then evaluated and these results suggested that SNEDDS with single hydrophilic surfactant (F45) and HLB 15 was the best formula for protein template as the stability testing showed that phase separation and precipitation did not appear. It was robust to pH and dilution with percentage of transmittance of 96.40±1.05% and the droplet size of 180.9nm. F45 had also uniform distribution of droplets size since the polydispersity index was less than 0.1. The zeta potential of F45 was -0.12mv with loading efficiency 83.57±1.77%. The emulsifying time of F45 was > 2min due to the formation of crystalline gel that was difficult to disperse. Key words: SNEDDS, BSA, hydrophllic surfactant, lypophilic surfactant, protein delivery
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