Marmara Pharmaceutical Journal of Research in Pharmacy Journal of Research in Pharmacy 2021 , Vol 25 Issue 3

Title	Marmara Pharmaceutical Journal of Research in Pharmacy Journal of Research in Pharmacy 2021 , Vol 25 Issue 3
Edition	Vol 25 Issue 3
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GMD	Karya Tulis Ilmiah
Language	Indonesia
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Abstract/Notes	ABSTRACT: The fractions (Fr.1-5) of the hydromethanolic extract of Abies numidica cones were studied for their polyphenol constituents, antioxidant, and anticholinesterase activities. The β-carotene-linoleic acid, cupric reducing power (CUPRAC), DPPH scavenging, and ABTS radical scavenging assays were used to determine antioxidant activity. Fr.3 exhibited the highest antioxidant activity in ABTS test (IC50: 1.29 μg/mL), b-carotene-linoleic acid test (IC50: 18.6 μg/mL) and CUPRAC (A0.5: 33.8 μg/mL) assays. Three fractions (Fr.2-4) promising antioxidant activity were analyzed using LC-MS/MS for their phenolic compositions. Taxifolin (155.9-2816.2 μg analyte/g extract), hyperoside (353.0- 2045.5 μg/g), vanillin (1488.9-1529.9 μg/g), tannic acid (1281.6-1416.8 μg/g), rosmarinic acid (1063.1-1149.3 μg/g), coumarin (683.0-772.6 μg/g), quercetin (881.0 μg/g), and catechin (277.7-400.2 μg/g) were identified in the antioxidant fractions. Moreover, the anticholinesterase activity was also performed using the in vitro spectroscopic Ellman method. The antioxidant fractions exhibited moderate butyrylcholinesterase inhibitory activity. The multi-ware analysis was performed to understand the origin of bioactivities. According to principal component analysis, it is detected that the hyperoside, catechin, taxifolin, and tannic acid were responsible for the antioxidant activity. Therefore, the cones of Abies numidica can be considered as potent antioxidant and anticholinesterase sources in food and pharmaceutical industries, commercially. KEYWORDS: Abies numidica; LC-MS/MS; antioxidant activity; anticholinesterase activity; taxifolin; hyperoside.
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